Determination of Risperidone and 9-hydroxyrisperidone in Human Plasma
A highly sensitive, selective, accurate and precise method for simultaneous quantification of risperidone and 9-hydroxyrisperidone in human plasma was developed. The method was based on electrospray ion trap tandem mass spectrometry in multiple reaction monitoring mode. Risperidone, 9-hydroxyrisperidone and carbamazepine, an internal standard, were extracted from plasma by a single step extraction with ether after the addition of sodium hydroxide and sodium chloride. A 5-μL aliquot of the sample reconstituted in mobile phase was analysed on a C18 column at 30 ˚C with the mobile phase consisting of acetonitrile and ammonium acetate buffer pH 5.5 (35:65 v/v).
Food Ingredients
The benefits of analysis using triple detection SEC chromatography
The ability to accurately determine the molecular weight distribution and branching characteristics of complex food ingredients is paramount. The performance, and in some cases the safety, of food additives based on polysaccharides depends strongly on their molecular weight and structure. Many polysaccharides used as food ingredients have large molecular sizes, however, and this can present a challenge for characterization.
Optimisation of Existing Methods for Analysis of Antibiotics in Meat using MS/MS Detection
The accurate detection and analysis of residual antibiotics in meat for human consumption is of critical importance in ensuring strict adherence to regulatory requirements for food safety. This article – the first in a series of features considering the detection of contaminants in meat – describes the optimization of an existing detection methodology that results in increased throughput by 5–10-fold, and more accurate and reproducible results, ensuring the best possible quantitation.