A simple-to-use slide-by-slide video presentation with instructional voiceover, together with a comprehensive handout supporting the learning experience.
This 45 minute video lecture outlines the basic operational principles of an electrospray interface (ESI) as used for LC/MS analyses. While we often feel that “theory” is a waste of time and we ask instead for “practical” information, this course will satisfy both of those needs. Appreciating how this very complex interface works will help the analyst to better understand the various (and numerous!), settings that must be dealt with to optimize an LC/MS method. A basic familiarity with electrospray operating principles will also make it clear why certain HPLC mobile phase compositions and components are best for LC/MS.
Who Should Take the Course?
Anyone who uses electrospray on a day-to-day basis and especially those responsible for method development; laboratory supervisors whose responsibilities include managing and approving analytical results from electrospray; persons considering the purchase of their first LC/MS system; any chemistry professional who wants to have a better understanding of the very important technique of electrospray.
How Will You Benefit from the Course?
You will:
- Gain a more complete understanding of the operation of the electrospray interface
- Have a “tool kit” for solving ESI problems
- Be better able to discuss electrospray interfaces with vendors and service personnel
- Have a foundation for learning detailed method development and optimization procedures
Instructor
Fred Klink, Mass-Spec-Training.com
Fred received a degree in biochemistry from Northwestern University and completed graduate studies in forensic chemistry at the University of Illinois. He spent seventeen years in the analytical instrument business where he held positions as an applications chemist, development project manager, product manager, marketing manager, and manager for strategic planning. Fred has been teaching highly regarded courses in mass spectrometry, solid-phase extraction, and protein analysis since 1996.
Fred's specialty is HPLC and SPE technologies and the application of mass spectrometry to protein characterization. Fred is the author of several journal articles and two book chapters: one on LC/MS for the Encyclopedia of Analytical Chemistry (Wiley, 2010) and another on chromatography systems in Cunico, Gooding, And Wehr, Basic HPLC and CE of Biomolecules (BBL, 1998). Fred is also a principal contributor to the monthly column MS Solutions. He is a member of the American Chemical Society and American Society for Mass Spectrometry.
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