A simple-to-use slide-by-slide video presentation with instructional voiceover, together with a comprehensive handout supporting the learning experience.
Perhaps more than any other analytical instrument, electrospray (ES) LC/MS method development requires the chemist to carefully proceed in a stepwise fashion and evaluate the results at each step. This 45 minute video lecture will provide you with the basic information needed to begin successful method development for ES.
Who Should Attend
Anyone who uses electrospray on a day-to-day basis and especially those responsible for method development and method validation; laboratory supervisors whose responsibilities include scheduling the development and validation of new methods and/or approving analytical results from electrospray; chemists who must review LC/MS data from an outside contract laboratory; any chemistry professional who wants to have a better understanding of the very important technique of electrospray.
How You will Benefit from this Course
- See the complete list of variables which effect ES LC/MS results
- Understand the important concept of ion suppression, it’s causes and solutions
- Learn about metal adduct formation and what to do about it
- Gain insights into how the choice of mobile phase constituents for ES analyses must be made depending on the analyte compound and analytical goal of the method
- Be better able to discuss electrospray results with colleagues, contract labs, and instrument service/support personnel
Instructor: Fred Klink, Mass-Spec-Training.com
Fred received a degree in biochemistry from Northwestern University and completed graduate studies in forensic chemistry at the University of Illinois. He spent seventeen years in the analytical instrument business where he held positions as an applications chemist, development project manager, product manager, marketing manager, and manager for strategic planning. Fred has been teaching highly regarded courses in mass spectrometry, solid-phase extraction, and protein analysis since 1996.
Fred's specialty is HPLC and SPE technologies and the application of mass spectrometry to protein characterization. Fred is the author of several journal articles and two book chapters: one on LC/MS for the Encyclopedia of Analytical Chemistry (Wiley, 2010) and another on chromatography systems in Cunico, Gooding, And Wehr, Basic HPLC and CE of Biomolecules (BBL, 1998). Fred is also a principal contributor to the monthly column MS Solutions. He is a member of the American Chemical Society and American Society for Mass Spectrometry.
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Previous viewing of “How Does Electrospray Work?” is recommended for those who are new to LC/MS.