A simple-to-use slide-by-slide video presentation with instructional voiceover, together with a comprehensive handout supporting the learning experience.
sponsored by:
Richard Henry, Sigma Aldrich, presents the "HPLC System Optimization" course. This 14-minute online learning seminar covers why instrument optimization is needed to achieve the best performance and includes brief reviews of sample dispersion, components that create instrument bandwidth (IBW) or dispersion, and how to accurately measure IBW for a given flow rate. If you are regular user of HPLC then this short online tutorial is ideal as a refresher for improving your HPLC analytical skills.
What will you gain from the course?
An introduction to the components of the HPLC system which play a vital role in optimizing analytical methods for improved quality of results.
What does it cover?
"HPLC System Optimization" covers:• Why instrument optimization may be needed to achieve the best performance from any high efficiency column
• A brief review of how sample dispersion occurs as it flows through an HPLC system (column and instrument)
• Description of the components that create instrument bandwidth (IBW) or dispersion, and how to accurately measure IBW for a given flow rate
• How to lower the IBW value if necessary (showing quantitative dispersion values for good and poor instruments) • Why it is important to know and report the IBW for every HPLC instrument that is used for high-performance columns
About the Instructor
Richard Henry received his B.S. degree in Chemistry from Juniata College in 1963 and Ph.D. in Analytical Chemistry from The Pennsylvania State University in 1966. After a postdoctoral year in separations at Purdue University with Professor L. B. Rogers, he joined DuPont in 1967 at the Experimental Station in Wilmington, DE and became one of the first employees of the DuPont Instrument Products Division in 1969. Dick worked closely with Dr. Jack Kirkland and others in the development of HPLC columns and packing materials while at DuPont. After about 10 years with Spectra-Physics and consulting on the west coast, he joined the Penn State University chemistry faculty in 1985 where he was Director of Analytical Laboratories and taught Instrumental Analysis to chemistry majors. In 1985, he also founded Keystone Scientific, Inc. where he developed HPLC columns and related separation technology. He retired from both Penn State University and Keystone Scientific in 2002, and remains active teaching short courses on separation technology and as a consultant. Dick has research interests in separation mechanisms and all applications of new LC column technology. He is also interested in new analytical techniques, especially the rapidly growing fields of UHPLC, chiral HPLC, LC-MS, and multidimensional separation methods. He served two terms as Chairman of the ACS Subdivision on Separations (1998-2002) and has also served for many years on its Executive Committee.
To access the e-Learning Module:1. Complete the right-hand registration form below in full and click 'Register'.2. Carefully read the 'Copyright Information' on the following page and if happy to proceed use the two buttons at the bottom of the page for access. Click 'View Seminar' to start the e-Learning Module and 'Download Handout' to download the related seminar materials. You will have unlimited access to this e-Learning Module for 1 month from registration. For subsequent access session simply return to the login page and input your email address and password.