Separation Science and John Dolan offer a LC-MS for Chromatographers workshop in The Thistle – Barbican, 120 Street Central, London, EC1V 8DS.
LC-MS for Chromatographers (7-8 October, 2010)
Price: EU
795 (for 2 day, including coffee/tea, lunch and all workshop materials)
15% DISCOUNT IF BOOKED WITH 'HPLC ADVANCED METHOD DEVELOPMENT' WORKSHOP
Who should take this course?
This course is for chromatographers who need to gain a thorough understanding of LC-MS. Practical applications to the analysis of drugs and metabolites in biological extracts are looked at. No previous experience of HPLC is necessary, although the course will be especially valuable to those with some chromatography experience.
What does it cover?
This two-day course explains the principles of mass spectrometric detection, the design and operation of mass spectrometers, and the interfacing of the MS to the liquid chromatograph to obtain both qualitative and quantitative data:
• MS operation, including operation of the most popular LC-MS interfaces
• How a quadrupole mass filter works
• MS calibration and optimization
• Ion production, fragmentation and detection
• Operation in MS, MS-MS and MS-MS-MS modes
• Structure determination by product ion analysis
• Quantitative analysis
• Method development and validation
• Techniques to get the most from your LC-MS
What will I get from the course?
You will learn how a mass spectrometer works, the advantages (and limitations) of adding mass spectrometric detection to your liquid chromatographic analyses, and how to use MS to approach separation and detection problems intractable by other methods. This course will give you a solid grounding on which to further build your LC-MS expertise.
Course Outline
Day 1
Overview of LC-MS
Principles of Mass Analysers
Basics of Quadrupole
Instrumentation
The Molecular Ion
Tuning and Calibration
Qualitative Analysis
Day 2
Developing the MS Method
Quantitative Analysis
Developing the LC Method
Sample Preparation
Validation
To register for this workshop, simply complete the form below: